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Human Coagulation

Zymogens

Human Prothrombin (Factor II)

Catalogue Number: HP 1002
Size: 9.0 mg

Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000 and consists of a single polypeptide chain. Activation of Human Prothrombin by Human Factor Va, Human Factor Xa and phospholipids yields the serine protease Human Alpha Thrombin. Human Prothrombin purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

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Human Prothrombin Fragment 1

Catalogue Number: F1
Size: 1.0 mg

Prepared from fresh frozen human plasma. Human Prothrombin Fragment 1 is a glycoprotein of molecular weight 21,700 and consists of a single polypeptide chain.

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Human Prothrombin Fragment 2

Catalogue Number: F2
Size: 1.0 mg

Prepared from fesh frozen human plasma. Human Prothrombin Fragment 2 is a glycoprotein of molecular weight 12,866 and consists of a single polypeptide chain.

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Human Factor VII

Catalogue Number: HFVII 1007
Size: 0.1 mg

Prepared from fresh frozen human plasma. Human Factor VII is a single chain vitamin K-dependent glycoprotein found in trace quantities in plasma (0.5 mg/Liter). In the Tissue Factor Pathway of coagulation, Human Factor VIIa, in the presence of calcium ions and tissue factor, activates Human Factor IX and Human X to their enzymatically active forms, Human Factor IXa and Human Factor Xa. Purity of Human Factor VII is determined by SDS-PAGE.

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Human Protein C

Catalogue Number: HPC 1001
Size: 0.1 mg

Human Protein C is purified from fresh frozen human plasma using a combination of salt precipitations and column chromatography. The Human Protein C purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Human Protein C is activated to the serine protease, Human Activated Protein C (APC) by Human Thrombin or the complex of Human Thrombin / Human Thrombomodulin and is a potent anticoagulant through the selective inactivation of Human Factor Va and Human Factor VIIIa.

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Human Factor IX

Catalogue Number: HFIX 1009
Size: 0.5 mg

Prepared from fresh frozen human plasma. The protein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa.

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Human Factor X

Catalogue Number: HFX 1010
Size: 0.8 mg

Prepared from fresh frozen human plasma. The purity of this Vitamin K-dependent protein is determined by SDS-PAGE gels and shows total reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor X, once activated via the Contact Factor Pathway or Tissue Factor Pathway, is responsible for the conversion of Prothrombin to Thrombin.

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Human Factor XI

Catalogue Number: HFXI 1111
Size: 0.1 mg

Prepared from fresh frozen human plasma. Human Factor XI is a glycoprotein of molecular weight 160,000. This protein migrates as a single band on 6 % SDS-PAGE gels in the absence of reducing agents. Human Factor XI is comprised of two identical polypeptide chains linked by disulphide bonds which yield a single band at 80,000 upon reduction with 2-mercaptoethanol. The specific clotting activity of purified Human Factor XI is 180-200 units/mg. In vivo, Factor XI is activated to Factor XIa by Factor XIIa. The resulting Human Factor XIa consists of two heavy and two light chains held together by disulphide bridges.

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Human Factor XII

Catalogue Number: HXII 1212
Size: 0.5 mg

Purified from fresh frozen human plasma. Human Factor XII is part of the contact activation system. Human Factor XII is a single chain glycoprotein with a molecular weight of approximately 80,000. Once activated, principally from the action of Kallikrein, Factor XII is converted into the active serine protease (Factor a-XIIa) that functions in the in vivo initiation of blood coagulation, fibrinolysis and kinin formation. Human Factor XII has been purified by ion exchange chromatography. Protein purity is determined by SDS-PAGE and activity is determined via clotting assay.

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Human Factor XIII

Catalogue Number: HFXIII 1313
Size: 0.25 mg

Human Factor XIII is a tetramer composed of two pairs of chains held together by non-covalent bonds. After activation of the zymogen via Thrombin to its active enzyme form, Factor XIIIa is responsible for catalyzing the formation of covalent bridges between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is very insoluble and resistant to lysis. Enzyme Research Laboratories offers the zymogen form of Human Factor XIII (a2b2).

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Human Prekallikrein

Catalogue Number: HPK 1302
Size: 1.0 mg

Purified from fresh frozen human plasma. Human Prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contract activation, kinin formation and fibrinolysis. Prekallikrein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay.

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Single Chain High Molecular Weight Kininogen

Catalogue Number: HK 1300
Size: 1.0 mg

High Molecular Weight Kininogen (HK) is a non-enzymatic cofactor of the contact activation system. HK is thought to have two functions in the contact activation system. First, HK links Prekallikrein to a negatively charged surface thereby allowing activation of Kallikrein by surface bound Factor a-XIIa. HK also forms a complex with Factor XI and accelerates its activation to XIa by a-XIIa. Additionally HK serves as a source of Bradykinin, a potent vasoactive peptide important in hypotension studies. The protein purity is determined by SDS-PAGE.

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Two Chain High Molecular Weight Kininogen

Catalogue Number: 2HK 1301
Size: 1.0 mg

ERL offers the two chain Kinin-free form of Kininogen. This is prepared by Kallikrein digestion of Kininogen which is then repurified to remove traces of Kallikrein.

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Human Glu-Plasminogen

Catalogue Number: HPG 2001
Size: 1.0 mg

Purified from fresh frozen human plasma. The protein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. No plasmin activity is detected using the chromogenic substrate S-2251. Plasminogen is activated to the serine protease Plasmin via urokinase, streptokinase or tissue plasminogen activator.

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Human Lys-Plasminogen

Catalogue Number: LPG 2002
Size: 1.0 mg

Purified from homogeneous Glu-Plasminogen by activation with Plasmin. This activation results in the release of a 76 amino acid peptide (Glu-Lys76). This Lys 77- Plasminogen can be readily converted to Lys 77-Plasmin by any of the common plasminogen activators. The protein purity is determined by SDS-PAGE.

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Enzymes

Human Alpha Thrombin (Factor IIa)

Catalogue Number: HT 1002a
Size: 1000 NIH U

Prepared from Human Prothrombin by activation with Human Factor Xa, Human Factor Va and phospholipid. Human Alpha Thrombin purity is determined by SDS-PAGE. Human Alpha Thrombin has a minimum activity of 2,700 NIH units/mg when compared to NIH standard Human Alpha Thrombin (WHO 2nd International Standard for Thrombin 01/580).

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Human Gamma Thrombin

Catalogue Number: HGT
Size: 1.0 mg

A non-clotting derivative of Thrombin produced from human alpha Thrombin by controlled digest with Trypsin-Sepharose. Gamma Thrombin is a non-coagulant form of Thrombin that retains much of its platelet activating capacity. Gamma Thrombin purity is determined by SDS-PAGE.

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Human Factor VIIa

Catalogue Number: HFVIIa
Size: 0.1 mg

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor XIIa is removed using affinity chromagtography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay. Factor VIIa, in the presence of calcium ions and the Tissue Factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

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Human Activated Protein C

Catalogue Number: APC
Size: 0.1 mg

Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen, Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.

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Human Factor IXa,b

Catalogue Number: HFIXa 1080
Size: 0.5 mg

Prepared from Human Factor IX by activation with Bovine Factor XIa. This Bovine Factor XIa is removed after activation. Complete activation is observed by SDS-PAGE. The Factor XIa activates Factor IX in a two step reaction. In the first step an internal Arg-Ala bond is cleaved and in the second step an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce Factor IXa,b.

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Human Factor Xa

Catalogue Number: HFXa 1011
Size: 0.8 mg

Human Factor Xa is prepared from homogeneous Human Factor X by activation with Russell's Viper Venom (RVV-X). This RVV-X is removed after activation. Complete activation is observed on 10 % SDS-PAGE gels. Factor Xa as part of the prothrombinase complex along with Factor Va, phospholipids and calcium ions catalyze the rapid conversion of Prothrombin to Thrombin.

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Human Factor Xa beta

Catalogue Number: HFXab
Size: 0.8 mg

Human Factor Xa beta

***NOT AVAILABLE***

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Human Factor XIa

Catalogue Number: HFXIa 1111a
Size: 0.1 mg

Prepared from Human Factor XI using Human Factor XIIa. This XIIa is removed using a corn trypsin inhibitor column. Complete activation is observed by SDS-PAGE. Factor XI, through the contact factor pathway cascade, is activated to Factor XIa via Factor XIIa and High Molecular Weight Kininogen. During activation by Factor XIIa and HK, FXI undergoes proteolytic cleavage in which the Mr=80,000 chain reportedtly is cleaved to a heavy and light chain with Mr of about 48,000 and 33,000. This Factor XIa is responsible for the activation of Factor IX to Factor IXa. Unlike other examples of activation of vitamin K-dependant blood-clotting proteins, Factor XIa proteolysis of Factor IX does not require membrane surfaces.

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Human Factor Alpha XIIa

Catalogue Number: HXIIa 1212a
Size: 0.5 mg

Human Factor a-XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. Human Factor XII and Prekallikrein are thought to be involved in a reciprocal activation mechanism in which XIIa activates Prekallikrein to Kallikrein which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. Enzyme Research Laboratories offers Factor a-XIIa which is activated by the autoactivation process with Dextran Sulphate and re-purified to remove the activator. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.

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Human Factor XIIa beta

Catalogue Number: HFXIIab
Size: 0.1 mg

Human Factor XIIa beta.

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Human Factor XIIIa

Catalogue Number: HFXIIIa 1314
Size: 0.25 mg

Prepared from purified Human Factor XIII. Human Factor XIII is cleaved with human alpha thrombin.  The thrombin is subsequently removed via chromatography. 

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Human Kallikrein

Catalogue Number: HPKa 1303
Size: 1.0 mg

Activation of Prekallikrein with Factor a-XIIa produces the enzymatically active Kallikrein. Kallikrein is a serine protease which consists of a heavy chain (molecular weight 52,000) and light chains (molecular weights either 36,000 or 33,000) linked by disulphide bridges. Kallikrein possesses enzymatic activity towards Factor XII, High Molecular Weight Kininogen, Plasminogen, Factor XI, Factor IX, Factor VII, prorenin and the complement system. After activation, the activating enzyme Factor XIIa is removed by affinity chromatography. Human Kallikrein purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.

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Human Plasmin

Catalogue Number: HPlasmin
Size: 1.0 mg

Prepared from Glu-Plasminogen using urokinase-bound activation column chromatography. Purity is judged by SDS-PAGE. Plasmin is a two chain serine protease linked by 2 disulfide bonds. Among other roles, Plasmin is responsible for the lysis of the fibrin clot, thus producing fibrin degradation products. (FDP's)

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Cofactors

Human Protein S

Catalogue Number: HPS
Size: 1.0 mg

Human Protein S exists in two forms in human plasma, as the free protein and in complex with Human C4b-binding protein. Enzyme Research Laboratories offers the free Human Protein S from plasma which serves as a cofactor for the anticoagulant activity of Human Activated Protein C. Human Protein S is a single chain glycoprotein with a molecular weight of 69,000. Human Protein S purity is determined by SDS-PAGE.

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Human Protein Z

Catalogue Number: HPZ
Size: 0.5 mg

The human protein Z is purified from plasma via a combination of precipitations and column chromatography. HPZ purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

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Human Fibronectin

Catalogue Number: FN
Size: 1.0 mg

At 300 ug/ml, plasma fibronectin is second to fibrinogen in abundance. It is a glycoprotein, composed of two subunits of approximately 220 kD each and participates in several cell-substratum adhesive interactions. Fibronectin binds to collagen, fibrinogen, fibrin, heparin. Enzyme Research Laboratories offers fibronectin purified by gelatin affinity chromatography followed by heparin chromatography.

 

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Inhibitors

Human a2-Antiplasmin

Catalogue Number: a2-AP
Size: 0.1 mg

Human a2-Antiplasmin, also known as Human α2-Plasmin Inhibitor, is a plasma glycoprotein that is a member of the SERPIN family of proteinase inhibitors. Human a2-Antiplasmin is the primary fast-acting inhibitor of plasmin in vivo, but has also been reported to inhibit other enzymes such as trypsin, elastase and Human Activated Protein C. Human a2-Antiplasmin is purified from normal human plasma using immunoaffinity and ion-exchange chromatography. Purified Human a2-Antiplasmin activity is determined by titration with purified plasmin.

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Human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)

Catalogue Number: TAFI
Size: 0.1 mg

Thrombin Activatable Fibrinolysis Inhibitor (TAFI), also known as plasma procarboxypeptidase-B and carboxypeptidase-U, is a single chain glycoprotein with a mass of 58,000 daltons. TAFI is purified from normal human plasma using immunoaffinity and ion-exchange chromatography.

 

***NOT AVAILABLE***

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Human Antithrombin

Catalogue Number: HAT
Size: 1.0 mg

Purified from fresh frozen human plasma. AT is a single chain glycoprotein which is considered to be the main physiological inhibitor of Thrombin and Factor Xa. In the presence of heparin, the inhibition of Thrombin and Factor Xa is enhanced from 300 to 1,000 fold. AT purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

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Human Heparin Cofactor II (HCII)

Catalogue Number: HCII
Size: 0.5 mg

Prepared from fresh frozen human plasma. Heparin Cofactor II is a single chain glycoprotein with a MW of 65,600 daltons. HCII is a specific inhibitor of Thrombin with increased activity in the presence of heparin. HCII purity is determined by SDS-PAGE and the activity is measured by the ability to inhibit Thrombin in the presence of saturating concentrations of heparin.

Product not currently available. Please inquire.

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Hirudin (HV-1)

Catalogue Number: Hir
Size: 0.1 mg

Prepared as a recombinant protein with Tyr-63 not sulphated. The 65 amino acid polypeptide is homogeneous as judged by 20% SDS-PAGE. Hirudin reacts rapidly with Thrombin to form a tight complex with a dissociation constant in the femtomolar range.

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Corn Trypsin Inhibitor

Catalogue Number: CTI
Size: 1.0 mg

Corn Trypsin Inhibitor (CTI) is a specific and potent inhibitor of Human Factor XIIa. It is purified from fresh Indiana sweet corn by solvent extraction and chromatographic methods. A suggested inhibitory concentration is 25 fold molar excess over the enzyme concentration. CTI purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

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Platelet & Plasma Proteins

Human Platelet Factor 4 (PF4)

Catalogue Number: PF4
Size: 0.1 mg

Platelet Factor 4 is purified from Human platelets by affinity chromatography and gel filtration. Platelet Factor 4 has a molecular weight of 29,000 daltons and is made up of 4 identical subunits of 7,800 daltons each.

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Human Apolipoprotein-H (B2-Glycoprotein-1)

Catalogue Number: HAPO-H
Size: 0.1 mg

B2Glycoprotein-1 (B2GP-1), also known as Apolipoprotein-H is a plasma glycoprotein that circulates at a concentration of 200 ug/ml (4 uM). B2GP-1 has been identified as a constituent of chylomicrons, very low density lipoproteins and high density lipoproteins in human plasma. It has also been demonstrated to bind phospholipids, heparin and platelets where it can modulate the activity of adenylate (19%). Although the precise function(s) are as yet unknown, B2GP-1 has been shown to interfere with blood coagulation by competitively binding to phospholipids exposed during cell activation or damage. Recent evidence also implicates B2GP-1 as a cofactor recognized by some anti-phospholipid antibodies present in autoimmune disorders such as systemic lupus erythematosus (SLE).

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Human GPIIbIIIa

Catalogue Number: GP2b3a
Size: 1.0 mg

Platelet membrane glycoproteins are involved in platelet adhesion and aggregation. Glycoproteins IIb and IIIa (GPIIb and GPIIIa) constitute the fibrinogen receptor and are required for platelet aggregation. Enzyme Research Laboratories offers GPIIbIIIa purified from human platelets. Glycoprotein IIb consists of 2 disulfide-linked subunits GPIIb, (MW = 125,000) and GPII (MW 23,000) while GPIIIa has only one polypeptide chain (MW 108,000), reduced). Human GPIIbIIIa migrates on gels as follows: GPIIb 136,000 non-reduced and 125,000 reduced. GPIIIa is 97,000 non-reduced and 108,000 reduced. Protein concentration is determined by the Bradford method.

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Fibrinogen

Human Fibrinogen, Plasminogen Depleted

Catalogue Number: FIB 1
Size: 1.0 gm

Purified from fresh frozen plasma. Human Fibrinogen, Plasminogen Depleted is homogeneous on 4-20 % SDS-PAGE gels and is greater than 95 % clottable. No other coagulation factors are detected by functional assay. Conversion of soluble Fibrinogen to the insoluble clot-forming fibrin is the terminal stage of blood coagulation.

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Human Fibrinogen, von Willebrand Factor and Plasminogen Depleted

Catalogue Number: FIB 2
Size: 1.0 gm

Purified from fresh frozen plasma, this protein has been purified using a combination of salt precipitations, affinity and immunoaffinity resins to remove Plasminogen and von Willebrand Factor. In a functional assay this protein is greater than 95 % clottable and is homogeneous on 4-20 % SDS-PAGE gels.

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Human Fibrinogen, Plasminogen, von Willebrand Factor and Fibronectin Depleted

Catalogue Number: FIB 3
Size: 1.0 gm

Purified from fresh frozen plasma, this protein has been purified using a combination of salt precipitations, affinity and immunoaffinity resins to remove Plasminogen, von Willebrand Factor and Fibronectin. In a functional assay this protein is greater than 95 % clottable and is homogeneous on 4-20 % SDS-PAGE gels.

(Product is packaged as 4 x 0.25 gm vials)

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Human Peak '1' Purified Fibrinogen (Factor XIII free)

Catalogue Number: P1FIB (XIII free)
Size: 10.0 mg

Human Peak '1' Fibrinogen accounts for 85% of the total plasma Fibrinogen. It contains mostly intact Aα subunit chains, is free of plasminogen, factor XIII, fibronectin, and other non-fibrinogen protein contaminants. Peak '1' Fibrinogen molecules each contain two copies of the platelet-binding γA chain C-terminal sequence and are ideal substrates for factor XIII assay.

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Human Peak '2' Purified Fibrinogen

Catalogue Number: P2FIB
Size: 10.0 mg

Human Peak '2' Fibrinogen accounts for 15% of the total plasma fibrinogen. It contains measurable amonts of factor XIII activity because the factor XIII B subunits bind to the γ chains and bring catalytic A subunits along with them. Thus Peak '2' fibrinogen serves as a 'carrier' for factor XIII in blood. The γ chain lacks the platelet recognition sequence but like all γ chains becomes crosslinked to dimers by factor XIIa.

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