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Human Factor VIIa

Catalogue Number: HFVIIa
Size: 0.1 mg

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor XIIa is removed using affinity chromagtography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay. Factor VIIa, in the presence of calcium ions and the Tissue Factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

Buffer Composition = 0.03 M Tris-HCl / 0.15 M NaCl / 0.1% PEG 8000 / 4% Ficoll Type 400 / pH 7.4

Extinction Coefficient (1%) = 13.9

Molecular Weight = 50,000 daltons

 

Supplied LYOPHILIZED. Shipped at ambient temperature. 

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